A preliminary study on L-asparaginase from mangrove detritus-derived fungi and its application in plant growth promotion
Varada S. Damare*, Kiran G. Kajawadekar
Affiliation
Department of Microbiology, Goa University, Taleigao Plateau, Goa 403206, India
*Corresponding author, email: [email protected]
Abstract
L-asparaginase is an enzyme with wide applications in medicine, the food industry, and the development of biosensors. However, its potential as a plant growth promoter remains poorly studied. In this preliminary study, we investigated the plant growth-promoting characteristics of L-asparaginase derived from two filamentous fungi, Peroneutypa sp. GU-S and Lecanicillium sp. GU-G, isolated from mangrove detritus in Goa, India. The enzyme produced by these fungi was active at room temperature and 37°C and showed higher activity under aeration. The optimal pH for asparaginase activity was determined to be 8. The enzyme from Peroneutypa sp. GU-S exhibited greater stability at lower pH (4) and was further processed. Economical sources, such as powdered soya and grass, were used for enzyme production via solid-state fermentation. Germinating seeds of pea (Pisum sativum) inoculated with the crude enzyme extract displayed enhanced seedling quality compared to un-inoculated seeds. Within twenty days of incubation, the shoot and root lengths of the test plants were 1.2 and 2.2 times greater, respectively, than those of the control plants. This study demonstrates the plant growth-promoting ability of L-asparaginase and encourages further investigation on other plant species.
Citation
Damare VS, Kajawadekar KG (2020) A preliminary study on L-asparaginase from mangrove detritus-derived fungi and its application in plant growth promotion. MycoAsia 2020/04. https://doi.org/10.59265/mycoasia.2020-04
Submission History
Received: 04.10.2019
Accepted: 22.06.2020
Published: 22.06.2020
Editorial Team
Handling Editor: Dr. Sanjay K. Singh